Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP53

Cell type

Cell type Class
Bone
Cell type
Saos-2
Primary Tissue
Bone
Tissue Diagnosis
Osteosarcoma

Attributes by original data submitter

Sample

Alias
E-MTAB-1394:SAOS+p53EE
Broker name
ArrayExpress
Description
Protocols: SAOS cells were cultured in Dulbecco's modified Eagle's medium (Life Technologies) supplemented with 10 % fetal bovine serum (PAA) and penicillin/streptomycin (Life Technologies) using standard conditions and procedures. Cells were transduced with recombinant adenovirus containing p53 wildtype, p53 R181E mutant, p53 E180R mutant, p53 R181E_E180R double mutant or GFP as previously described (Cam et al., 2006). ChIP was perfomed with a p53-specific antibody. The DNA was purified with the Qiaquick PCR purification Kit (Qiagen).
INSDC center alias
University of Marburg
INSDC center name
University of Marburg
INSDC first public
2014-02-14T17:02:00Z
INSDC last update
2018-03-08T16:10:48Z
INSDC status
public
SRA accession
ERS197251
Sample Name
ERS197251
Title
SAOS+p53EE
cell line
SAOS
immunoprecipitate
Anti-p53, sc-126, Santa-cruz
transfection
EE

Sequenced DNA Library

library_name
SAOS+p53EE
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was perfomed with a p53-specific antibody. The DNA was purified with the Qiaquick PCR purification Kit (Qiagen).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
213903005
Reads aligned (%)
48.2
Duplicates removed (%)
73.3
Number of peaks
4803 (qval < 1E-05)

hg38

Number of total reads
213903005
Reads aligned (%)
48.5
Duplicates removed (%)
71.8
Number of peaks
5265 (qval < 1E-05)

Base call quality data from DBCLS SRA