Characterization of the p53 cistrome - DNA binding cooperativity dissects p53's tumor suppressor functions
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TP53
Cell type
Cell type Class
Bone
Cell type
Saos-2
Primary Tissue
Bone
Tissue Diagnosis
Osteosarcoma
Attributes by original data submitter
Sample
Alias
E-MTAB-1394:SAOS+p53wt
Broker name
ArrayExpress
Description
Protocols: SAOS cells were cultured in Dulbecco's modified Eagle's medium (Life Technologies) supplemented with 10 % fetal bovine serum (PAA) and penicillin/streptomycin (Life Technologies) using standard conditions and procedures. Cells were transduced with recombinant adenovirus containing p53 wildtype, p53 R181E mutant, p53 E180R mutant, p53 R181E_E180R double mutant or GFP as previously described (Cam et al., 2006). ChIP was perfomed with a p53-specific antibody. The DNA was purified with the Qiaquick PCR purification Kit (Qiagen).
INSDC center alias
University of Marburg
INSDC center name
University of Marburg
INSDC first public
2014-02-14T17:02:00Z
INSDC last update
2018-03-08T16:10:48Z
INSDC status
public
SRA accession
ERS197247
Sample Name
ERS197247
Title
SAOS+p53wt
cell line
SAOS
immunoprecipitate
Anti-p53, sc-126, Santa-cruz
transfection
p53wt
Sequenced DNA Library
library_name
SAOS+p53wt
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was perfomed with a p53-specific antibody. The DNA was purified with the Qiaquick PCR purification Kit (Qiagen).