Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H2AK5ac

Cell type

Cell type Class
Blood
Cell type
HPC-7
NA
NA

Attributes by original data submitter

Sample

Alias
E-MTAB-3954:H2A_AcK5_HPC7
Broker name
ArrayExpress
Description
Protocols: HPC-7 cells (Pinto do O et al., 1998) were grown in Stem Cell Factor. Hi-C and CHi-C was performed as described previously (Schoenfelder et al., 2015). Sequencing libraries were prepared according to the manufactures instructions.
ENA checklist
ERC000011
INSDC center alias
University of Cambridge
INSDC center name
University of Cambridge
INSDC first public
2016-02-29T17:01:41Z
INSDC last update
2018-03-09T09:21:13Z
INSDC status
public
SRA accession
ERS939102
Sample Name
ERS939102
Title
H2A_AcK5_HPC7
cell line
HPC-7
cell type
haematopoietic stem/progenitor cell
organism
Mus musculus
sequencing type
Hi-C sequencing

Sequenced DNA Library

library_name
H2A_AcK5_HPC7
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
HPC-7 cells (Pinto do O et al., 1998) were grown in Stem Cell Factor. Hi-C and CHi-C was performed as described previously (Schoenfelder et al., 2015). Sequencing libraries were prepared according to the manufactures instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 1000

mm10

Number of total reads
31893995
Reads aligned (%)
86.3
Duplicates removed (%)
6.7
Number of peaks
3141 (qval < 1E-05)

mm9

Number of total reads
31893995
Reads aligned (%)
86.2
Duplicates removed (%)
6.7
Number of peaks
3179 (qval < 1E-05)

Base call quality data from DBCLS SRA