Illumina HiSeq 2500 sequencing; Elucidation of FOXP1 target genes in DLBCL by ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
FOXP1
Cell type
Cell type Class
Blood
Cell type
U-2932
Primary Tissue
Blood
Site of Extraction
Ascites
Tissue Diagnosis
Lymphoma B-cell
Attributes by original data submitter
Sample
ENA first public
2015-12-01
ENA last update
2018-03-09
ENA-CHECKLIST
ERC000011
External Id
SAMEA3481926
INSDC center alias
Institute of Molecular Cancer Research, University of Zurich
INSDC center name
Institute of Molecular Cancer Research, University of Zurich
INSDC first public
2015-12-01T17:02:06Z
INSDC last update
2018-03-09T02:15:40Z
INSDC status
public
Submitter Id
E-MTAB-3741:U-2932 FOXP1 IP
broker name
ArrayExpress
cell line
U-2932
common name
human
disease
diffuse large B-cell lymphoma
immunoprecipitate
FOXP1 IP
sample name
E-MTAB-3741:U-2932 FOXP1 IP
sex
female
Sequenced DNA Library
library_name
U-2932 FOXP1 IP
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The DLBCL cell lines used were SUDHL6, RC-K8 and SUDHL4 of GCB DLBCL subtype and U-2932 of ABC DLBCL. Cell lines were maintained at 37 degrees C, 5%CO2 in a humidified atmosphere in RPMI supplemented with 20% heat-inactivated FBS and antibiotics. DNA was isolated using the NucleoSpin Gel- and PCR-cleanup Kit (Macherey Nagel). 1.5ng of DNA was amplified using the Ovation Ultralow Library System (NuGEN).