ChIP-seq study using a cell line model of ER AR+ molecular apocrine tumours (AR FoxA1 molecular apocrine)
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
ENA first public
2012-02-18
ENA last update
2018-03-08
External Id
SAMEA1094834
INSDC center alias
CRUK-CRI
INSDC center name
CRUK-CRI
INSDC first public
2012-02-18T17:00:29Z
INSDC last update
2018-03-08T15:36:05Z
INSDC status
public
Submitter Id
E-MTAB-986:MCF7
broker name
ArrayExpress
cell line
MCF7
cell type
epithelial
common name
human
organism part
breast
sample name
E-MTAB-986:MCF7
sex
female
Sequenced DNA Library
library_name
jc430
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
MDA-MB-453 and MCF7 cells were grown in DMEM containing 10% heat-inactivated FBS, 2 mM L glutamine, 50 U/ml penicillin and 50 _g/ml streptomycin and LNCaP cells were grown in RPMI containing 10% heat-inactivated FBS, 2 mM L glutamine, 50 U/ml penicillin and 50 _g/ml streptomycin. The antibodies used were anti-AR (sc-816) and anti-ER (sc-543) from Santa Cruz Biotechnologies and anti-FoxA1 (ab5089) from Abcam. Four 15 cm dishes of cells were used per chromatin immunoprecipitation (ChIP) and samples were processed according to standard ChIP procedures as described in Schmidt et al, Methods 48(3) July 2009 doi:10.1016/j.ymeth.2009.03.001. The immunoprecipitated DNA was subsequently amplified as previously described for Illumina sequencing (Schmidt et al, 2009).