Antigen

Antigen Class

No description

Antigen

NA

Cell type

Cell type Class

Blood

Cell type

Erythroid Cells

MeSH Description

The series of cells in the red blood cell lineage at various stages of differentiation.

Sample

ENA-FIRST-PUBLIC

2012-11-14

ENA-LAST-UPDATE

2013-11-04

External Id

SAMEA1563395

INSDC center alias

BI-UK

INSDC center name

The Babraham Institute, Cambridge, UK

INSDC first public

2012-11-14T17:01:58Z

INSDC last update

2013-11-04T20:04:57Z

INSDC status

public

Submitter Id

mouseTA_sample_0:0:4

common name

house mouse

sample name

mouseTA_sample_0:0:4

tissue

erythroid cells

Sequenced DNA Library

library_name

Erythroid RNA-PolII ChIP experiment

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNAPII ChIP was carried out as described in Mitchell and Fraser 2008. Genome-wide RNAPII association was determined by sequencing libraries constructed from the RNAPII-Pser5 chromatin immunopurified (using Ab5131, Abcam) and genomic input material. Library preparation was performed according to Illumina PE genomic protocol, incorporating improvements suggested in Quail et al 2008, with all reactions scaled according to starting DNA quantity.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

10854088

Reads aligned (%)

92.8

Duplicates removed (%)

8.7

Number of peaks

335 (qval < 1E-05)

mm9

Number of total reads

10854088

Reads aligned (%)

92.6

Duplicates removed (%)

9.3

Number of peaks

320 (qval < 1E-05)